Abstract
THE use of ultra-violet light to detect and photograph chromatographed compounds absorbing in the region of 254 mµ is well known1. In the course of investigating a number of unknown compounds found on chromatograms of plasma extracts, a simple technique was developed for photographing spots which fluoresce in ultra-violet light at 360 mµ. This consists of interposing a Kodak 2A gelatin filter, which absorbs radiant energy below 405 mµ, between the chromatogram and the contact paper (‘Kodabromide F5’). The filter is enclosed for protection between 1/16 in. glass plates, bound with tape at the edges. The chromatogram is exposed for approximately 30 sec. to ultra-violet light from a 365-mµ source (‘Mineralight’ handlamp, Ultraviolet Products, Inc., San Gabriel, Calif.) at a distance of 1 ft. The photograph is then developed in the usual way.
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References
Markham, R., and Smith, J. D., Biochem. J., 45, 294 (1949).
Smith, J. D., and Markham, R., Biochem. J., 46, 509 (1950).
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ABELSON, D. Ultra-Violet Photography of Fluorescent Spots on Chromatograms of Biological Extracts. Nature 188, 850–851 (1960). https://doi.org/10.1038/188850a0
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DOI: https://doi.org/10.1038/188850a0
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