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Isolation of Mutants of Bacillus subtilis requiring D-Glutamic Acid

Abstract

ALTHOUGH synthetic pathways of the L series of amino-acids have been studied extensively by the use of biochemical mutants of micro-organisms, there appears to be no published account of the synthesis of the D series of amino-acids using similar experimental techniques. Meanwhile, because D-glutamic acid is known to be an essential component of the cell wall and capsular fractions of a certain kind of bacteria1,2, an attempt to obtain mutants requiring D-glutamic acid for growth is still valuable. With this idea, induction and isolation of mutants from a wild strain of Bacillus subtilis K requiring D-glutamic acid was attempted.

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References

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MOMOSE, H., IKEDA, Y. Isolation of Mutants of Bacillus subtilis requiring D-Glutamic Acid. Nature 186, 567–568 (1960). https://doi.org/10.1038/186567b0

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