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Multiplication of Vaccinia Virus in Serum-free and Serum-containing Cell Cultures

Nature volume 185, pages 123–124 (1960)Cite this article

Abstract

THE use of tissue culture cells for the production of virus preparations and vaccines is increasing and will gain prominence in the future. A report has recently appeared concerning the possible use of cell cultures for the standardization of smallpox vaccines1. At present the cell lines used for the production of virus vaccines are grown in media which contain of necessity serum of one species or another2. Several cell lines which grow in media free of serum have been reported3, and the advantages that these cells might have for vaccine production are obvious. Their use, however, could well be limited by their ability to support virus growth to a high titre. This report describes a comparison of vaccinia growth in serum-free and serum-containing mouse lung cells.

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References

  1. Cutchins, E., and Warren, J., Proc. Soc. Exp. Biol. Med., 97, 456 (1958).

    Article  CAS  Google Scholar 

  2. Hilleman, M. R., et al., J. Amer. Med. Assoc., 163, 4 (1957). Salk, J. E., ibid., 158, 1239 (1955).

    Article  Google Scholar 

  3. McQunilkin, W. T., Evans, V. J., and Earle, W. R., J. Nat. Cancer Inst., 19, 885 (1957). Waymouth, C., ibid., 22, 1003 (1959). Pumper, R. W., Science, 128, 363 (1958).

    Google Scholar 

  4. Younger, J. S., Proc. Soc. Exp. Biol. Med., 85, 202 (1954).

    Article  Google Scholar 

  5. Morgan, J. F., Morton, H. J., and Parker, R. C., Proc. Soc. Exp. Biol. Med., 73, 1 (1950).

    Article  CAS  Google Scholar 

  6. Cassel, W. A., Virol., 3, 514 (1957).

    Article  CAS  Google Scholar 

  7. Sanford, K. K., Earle, W. R., and Likely, G. D., J. Nat. Cancer Inst., 9, 229 (1948).

    CAS  PubMed  Google Scholar 

  8. Reed, L. J., and Muench, H., Amer. J. Hyg., 27, 493 (1938).

    Google Scholar 

  9. Pumper, R. W., Amer. J. Hyg., 69, 79 (1959).

    CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology, College of Medicine, University of Illinois, Chicago

    ROBERT W. PUMPER, LAWRENCE J. ALFRED & DAVID L. SACKETT

Authors
  1. ROBERT W. PUMPER
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  2. LAWRENCE J. ALFRED
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  3. DAVID L. SACKETT
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PUMPER, R., ALFRED, L. & SACKETT, D. Multiplication of Vaccinia Virus in Serum-free and Serum-containing Cell Cultures. Nature 185, 123–124 (1960). https://doi.org/10.1038/185123a0

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