Abstract
ALTHOUGH the tocopherols, and in particular α-tocopherol, are widely distributed in Nature, they have not so far been identified in micro-organisms. The only micro-organism that has been adequately studied is bakers' yeast, which has been found to contain no tocopherol, although it contains another substance with vitamin E-like activity1. In view of recent work connecting α-tocopherol with some fundamental cellular processes, such as the reduced diphosphopyridine nucleotide-cytochrome c reductase system2, the regulation of succinate oxidation in rat liver mitochondria3 and the synthesis of adenosine triphosphate4, it was of interest to investigate the occurrence of tocopherols in a range of micro-organisms. The following organisms were grown in deep culture, using suitable media, the general composition of which is shown in Table 1: three strains of Esch. coli., 8196 and two vitamin B12-requiring mutants, N.I.R.D. and M200 (C181 strain) ; Streptococcus fœcalis R.; Bacillus cereus (strain 5) ; Staphylococcus aureus 4163; the nitrogen-fixing bacterium, Rhizobium leguminosarum 317 ; three sulphur-requiring, chlorophyll-containing bacteria, Chromatium 8379, Chlorobium thiosulphatophilum and Thiopedia; two chlorophyll-containing protozoa, Ochromonas malhamensis and Euglena gracilis; and one non-chlorophyll containing protozoan, Tetrahymena pyriformis.
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References
Forbes, M., Zilliken, F., Roberts, G., and György, P., J. Amer. Chem. Soc., 80, 385 (1958).
Nason, A., and Lehman, I. R., J. Biol. Chem., 222, 511 (1956).
Corwin, L. M., and Schwarz, K., J. Biol. Chem., 234, 191 (1959).
Gray, D. E., J. Vitaminology, 4, 172 (1958).
Green, J., Marcinkiewicz, and Watt, J. Sci. Food Agric., 6, 274 (1955).
Green, J., J. Sci. Food Agric., 9, 801 (1958).
Dam, H., and Glavind, J., Biochem. J., 32 I, 485 (1938).
Dam, H., Glavind, J., and Nielson, R., Z. physiol. Chem., 265, 80 (1940).
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GREEN, J., PRICE, S. & GARE, L. Tocopherols in Micro-organisms. Nature 184, 1339 (1959). https://doi.org/10.1038/1841339a0
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DOI: https://doi.org/10.1038/1841339a0
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