Abstract
ACETONE-HYDROCHLORIC acid has been widely used to split hæmoproteins, including cytochromes of the a type which contain formyl substituents in their prosthetic groups. Results reported below show that formyl porphyrins and hæmins react with acetone-hydrochloric acid, and hence it is necessary to exercise caution in the use of this reagent. Fortunately the reaction between formyl porphyrins and acetone-hydrochloric acid is a slow one (48–72 hr. being necessary for complete conversion at room temperature) so that if the temperature is kept low and only a relatively short time is allowed for cleavage of hæmoprotein the amount of alteration of a formyl group is negligible.
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CLEZY, P. Reaction of Formyl Porphyrins with Acetone-Hydrochloric Acid. Nature 184, 1142–1143 (1959). https://doi.org/10.1038/1841142b0
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DOI: https://doi.org/10.1038/1841142b0
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