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Separation and Isolation of Fractions of Rabbit Gamma-Globulin containing the Antibody and Antigenic Combining Sites

Nature volume 182, pages 670–671 (1958)Cite this article

Abstract

SOME years ago1 the digestion of rabbit anti-ovalbumin with papain powder was investigated, and it was found that a fragment could be obtained which would no longer form a precipitate with ovalbumin but which would specifically inhibit the precipitation of ovalbumin with undigested antiserum. This fragment, which presumably contained an antibody-combining site, was believed to have a molecular weight of 40–50,000, or about one-quarter that of the original γ-globulin. Earlier, Peterman2 had shown by ultracentrifuge studies that digestion of human γ-globulin with crude bromelin and papain produced fragments of one-half to one-quarter the size of the original molecule.

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References

  1. Porter, R. R., Biochem. J., 46, 479 (1950).

    Article  CAS  Google Scholar 

  2. Peterman, M. L., J. Amer. Chem. Soc., 68, 106 (1946).

    Article  Google Scholar 

  3. Kimmel, J. R., and Smith, E. L., J. Biol. Chem., 207, 515 (1954).

    CAS  PubMed  Google Scholar 

  4. Peterson, E. A., and Sober, H. A., J. Amer. Chem. Soc., 78, 751 (1956).

    Article  CAS  Google Scholar 

  5. Archibald, W. J., J. Phys. Chem. Soc., 78, 751 (1956).

    Google Scholar 

  6. Treffers, H. P., “Adv. Prot. Chem.”, 1, 69 (1944).

    Article  CAS  Google Scholar 

  7. Pauling, L., J. Amer. Chem. Soc., 62, 457 (1940).

    Article  Google Scholar 

  8. Porter, R. R., Biochem. J., 46, 473 (1950).

    Article  CAS  Google Scholar 

  9. McFadden, M. L., and Smith, E. L., J. Biol. Chem., 214, 185 (1955).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. National Institute for Medical Research, The Ridgeway, Mill Hill, N.W.7.

    R. R. PORTER

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  1. R. R. PORTER
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PORTER, R. Separation and Isolation of Fractions of Rabbit Gamma-Globulin containing the Antibody and Antigenic Combining Sites. Nature 182, 670–671 (1958). https://doi.org/10.1038/182670a0

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