Abstract
STARCH-GEL electrophoresis, as introduced by Smithies1, has proved superior to both free and filter-paper electrophoresis for separating protein mixtures2–6. However, the opacity of the strips excludes the possibility of direct quantitative evaluation of separated proteins, though this is possible with the other two electrophoretic methods. This problem was successfully solved recently by Fine and Waszczenko7, who prepared starch-gel suitable for direct quantitative evaluation by transmission photometry. A simpler and quicker method for obtaining transparency of the strips is presented here.
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References
Smithies, O., Biochem. J., 61, 629 (1955).
Ashton, G. C., Nature, 180, 917 (1957).
Latner, A. L., and Zaki Angelhabib, Nature, 180, 1366 (1957).
Poulik, M. D., Nature, 180, 1477 (1957).
Poulik, M. D., and Smithies, O., Biochem. J., 68, 636 (1958).
Smithies, O., and Poulik, M., Nature, 177, 1033 (1956).
Fine, J. M., and Waszczenko-Z., E., Nature, 181, 269 (1958).
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VESSELINOVITCH, S. A Simple Method for making Starch-gel Electrophoretic Strips Transparent. Nature 182, 665 (1958). https://doi.org/10.1038/182665a0
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DOI: https://doi.org/10.1038/182665a0
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