Abstract
RECENT methods for the assay of amylase use polysaccharides, mostly starch solutions, as a substrate. The wide range of the results can be ascribed in part to the properties of polysaccharides in solution. In starches the ratio of amylose to amylopectin is variable and, according to Meyer, long-chain polysaccharides in solution tend to form particles resistant to enzyme. The affinity of amylase for the substrate diminishes with decreasing chain-length. The maltose set free acts as an inhibitor of amylase (cf. Meyer et al.). Moreover, the moderate accuracy of the usual (iodometric and reducto-metric) methods of measuring chain-length may affect the results unfavourably.
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References
Meyer, K. H., and Gonon, W. F., Helv. Chim. Acta, 34, 308 (1951).
Pazur, J. H., J. Biol. Chem., 205, 75 (1953).
Pazur, J. H., and Budovich, T., Science, 121, 702 (1955).
Wheelan, W. J., and Roberts, P. J. P., J. Chem. Soc., 1298 (1953).
Svanborg, K., and Myrbäck, K., Arkiv Kemi, 6, 113 (1953).
Berger, H., thesis, Leyden (1956).
Myrbäck, K., and Willstaedt, E., Arkiv Kemi, 6, 465 (1953).
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JANSEN, A., WYDEVELD, P. α-(p-Nitrophenyl)Maltoside as a Substrate for the Assay of Amylase. Nature 182, 525–526 (1958). https://doi.org/10.1038/182525b0
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DOI: https://doi.org/10.1038/182525b0
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