Abstract
THIS method for the spectrophotometric determination of protein in alkaline solution was developed in order to determine the protein of Chlorella cells. It was not possible to obtain Chlorella-cell protein, readily, in acid solution. It was possible to break most of the cells by grinding mechanically with silica sand but this method is tedious and time-consuming. The latter point is important when it is necessary to measure physiological changes which proceed at a rapid rate.
Similar content being viewed by others
Article PDF
References
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 264 (1951).
Pirie, N. W., “Modern Methods of Plant Analysis”, 4, 23, ed. by Paech, K., and Tracey, M. V. (Springer-Verlag, Berlin, 1955).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HEWITT, B. Spectrophotometric Determination of Protein in Alkaline Solution. Nature 182, 246 (1958). https://doi.org/10.1038/182246a0
Issue Date:
DOI: https://doi.org/10.1038/182246a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.