Abstract
IN the study of protein metabolism it is often essential to be able to refer the incorporation of isotopic amino-acids to some individual type of protein, characterizable, for example, by means of a particular enzymatic activity or by its antigenic specificity1. As shown previously, soluble proteins derived from microscopic and submicroscopic fractions of liver cells can be characterized immunologically by means of precipitin reactions in agar plates2. In order to analyse some of the early pathways occurring in protein metabolism under various conditions, an attempt was then made to combine this method of protein identification with autoradiography in isotope incorporation experiments. A short account of the procedure and the results obtained are given in this communication3.
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References
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PERLMANN, P., HULTIN, T. Autoradiographic Analysis on Agar Plates of Labelled Antigens from Rat Liver. Nature 182, 1530–1531 (1958). https://doi.org/10.1038/1821530b0
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DOI: https://doi.org/10.1038/1821530b0
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