Abstract
JUST prior to spinning their cocoons, each of twenty silkworms was injected with 1.7 mgm. (25 µc.) of formate labelled with carbon-14. The fibroin, isolated by the method of Dunn et al. 1, was hydrolysed and the glycine isolated as nitronaphthalene sulphonate. This salt was recrystallized several times, then dissociated on a ‘Dowex’ 50 column; free glycine was eluted with 3 N ammonia and crystallized in water/ethanol/diethylether. Its chemical purity was checked by paper chromatography in two different solvents (phenol/isopropyl alcohol/water and butanol/acetic acid/water). The specific radioactivities of the nitronaphthalene sulphonate and of the free glycine remained constant throughout the recrystallizations.
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References
Dunn, M. S., Camien, M. N., Rockland, L. B., Shankman, S., and Goldberg, S. C., J. Biol. Chem., 155, 591 (1944).
Van Slyke, D. D., MacFadyen, D. A., and Hamilton, P., J. Biol. Chem., 141, 671 (1941).
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BRICTEUX-GRÉGOIRE, S., VERLY, W. Utilization of Formate for the Biosynthesis of Glycine Carbon-1 and -2 in Bombyx mori . Nature 182, 1515–1516 (1958). https://doi.org/10.1038/1821515b0
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DOI: https://doi.org/10.1038/1821515b0
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