Abstract
LIEBERMAN1 has recently described the difficulty experienced in obtaining from apples cytoplasmic particles having enzymatic activity; he found it necessary to homogenize his apple tissue in tris buffer at pH 9.2 in order to obtain particles with appreciable cytochrome oxidase activity. Hulme2 has previously found it necessary to use alkaline buffers to extract enzymes from apple tissue.
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References
Lieberman, M., Science, 127, 189 (1958).
Hulme, A. C., Nature, 158, 588 (1946).
Bonner, W. D., Plant Physiol., 30, xxx (1955).
Bonner, W. D., “Ann. Rev. Plant Physiol.”, 8, 427 (1957).
Lieberman, M., and Biale, J. B., Plant Physiol., 31, 420 (1956).
Neal, G. E., and Hulme, A. C., J. Exp. Bot., 9, 142 (1958).
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NEAL, G., HULME, A. Activity of Cytoplasmic Particles isolated from Apples. Nature 181, 1469–1470 (1958). https://doi.org/10.1038/1811469a0
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DOI: https://doi.org/10.1038/1811469a0
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