Abstract
ALTHOUGH the distribution of nitrogen-fixing Clostridia in soils is wide, difficulties are encountered with their quantitative estimation. Recently, Parker1 suggested that the ‘nitrogen-free’ media normally used may be deficient in essential growth factors and also showed that other media could inhibit development, although the toxicity of the media could be neutralized by raw potato sap. The influence of media is further investigated in the present study using Taupo light sandy loam, a low-fertility pumice soil under tussock grassland from Waiouru, New Zealand, and also a typical starch-fermenting strain of Clostridium butyricum isolated from this soil and shown to fix 4 mgm. nitrogen per gm. glucose utilized in the enriched media afterwards described.
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References
Parker, C. A., Austr. J. Agric. Res., 5, 90 (1954).
Swaby, R. J., Austr. J. Exp. Biol. Med. Sci., 17, 401 (1939).
Mackie, T. J., and McCartney, J. E., “Handbook of Practical Bacteriology” (E. and S. Livingstone, Ltd., Edinburgh, 1950).
Augier, J., Ann. Inst. Past., 92, 817 (1957).
Taylor, C. B., Nature, 168, 115 (1951).
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ROSS, D. Influence of Media on the Counts of Clostridium butyricum in Soils. Nature 181, 1142–1143 (1958). https://doi.org/10.1038/1811142a0
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DOI: https://doi.org/10.1038/1811142a0
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