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Carbon Dioxide as a Reversible Inhibitor of Spermatozoan Metabolism

Abstract

LOW-TEMPERATURE storage (+ 5° to − 79° C.) has been the primary means of preserving the life and fertility of mammalian spermatozoa in vitro. Experiments in our laboratory have repeatedly shown that low levels of carbon dioxide prolonged the life of bovine sperm at 37° C. under either aerobic or anaerobic conditions1. Recent experiments have shown that high concentrations of carbon dioxide in a properly buffered medium reversibly inhibit sperm motility and metabolism. Preliminary results have shown that the fertility of bovine semen stored for several days at room temperature in a diluent saturated with carbon dioxide is not impaired2.

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References

  1. Baker, F. N., VanDemark, N. L., Salisbury, G. W., and VanTienhoven, A., J. Animal Sci., 11, 788 (1952). Salisbury, G. W., and Kinney, jun., W. C. (unpublished results, 1953). Kinney, jun., W. C., M.S. thesis, University of Illinois (1954).

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  2. VanDemark, N. L., and Sharma, U. D., J. Dairy Sci., 40, 438 (1957).

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SALISBURY, G., VANDEMARK, N. Carbon Dioxide as a Reversible Inhibitor of Spermatozoan Metabolism. Nature 180, 989–990 (1957). https://doi.org/10.1038/180989a0

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