Abstract
IT has been reported that formate carbon-14 is preferentially taken up into deoxyribonucleic acid thymine by bone marrow cells in vitro 1,2. This has been further confirmed by the ‘thymidine dilution test’. Suspensions of human bone marrow cells were cultured in vitro 3, and the formate carbon-14 uptake was measured on high-resolution autoradiographs using the stripping film method on alcohol-fixed smears from such cultures4. The proportion of labelled cells (‘per cent positive’) was obtained by counting 250 cells, and the average count per cell by grain counts over 50 cells. The error-range of such counts is within ± 10 per cent. It was found that 0.18 mgm./ml. medium thymidine depressed formate carbon-14 uptake by 80 ± 11 per cent (seven experiments), while leaving adenine carbon-14 uptake unaffected (four experiments).
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LAJTHA, L. Lack of Differential Radiation Effect on the Incorporation of Labelled Precursors into Deoxyribonucleic Acid. Nature 180, 1048 (1957). https://doi.org/10.1038/1801048a0
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DOI: https://doi.org/10.1038/1801048a0
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