Abstract
IT has been found that, when leaves from near the apex of dried herbarium material of various leafy hepatics (Jungermaniales Aorogynae) are stained in acetocarmine, certain of the nuclear components are clearly visible. In Frullania squarrosa, female material thirty-three years old and male material two years old have been examined and found to show clearly the heteropycnotic sex chromosomes, heteropycnotic fragments of other chromosomes, and the nucleolus. All these features are also distinguishable after staining of fresh material. Since the sex chromosome mechanism in this species and in other species belonging to the subgenus Galeiloba is an X1-X2-Y mechanism 1,2, the sex of the material may be determined by counting the number of heteropycnotic chromosomes.
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References
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BERRIE, G. Cytological Examination of Herbarium Material of Hepatics. Nature 179, 1143 (1957). https://doi.org/10.1038/1791143a0
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DOI: https://doi.org/10.1038/1791143a0
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