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‘Kernechtrot’ or Nuclear Fast Red in the Histochemical Detection of Calcareous Corpuscles in Taenia saginata

Abstract

Taenia saginata, like many other cestodes1, is known to contain numerous calcareous corpuscles or calcium balls distributed throughout the parenchyma. These corpuscles, in addition to their calcium content, consist of an organic base, containing2 deoxyribo- and ribo-nucleic acids, simple proteins, glycogen and a hyaluronic acid - type of polysaccharide. With Gomori's modified technique these corpuscles also gave a positive reaction for alkaline phosphatase. The presence of calcium in these balls was indicated by von Kossa's silver test for calcium3.

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References

  1. von Brand, T., “Chemical Physiology of Endoparasitic Animals” (Acad. Press, Inc., New York, 1952).

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  2. Chowdhury, A. B., Dasgupta, B., Ray, H. N., and Bhaduri, N. V., Bull. Calcutta School Trop. Med., 3, 52 (1955).

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  3. Glick, D., “Techniques of Histo- and Cytochemistry” (Interscience Pub., New York and London).

  4. McGee-Russell, S. M., Nature, 175, 301 (1955).

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CHOWDHURY, A., DASGUPTA, B. & RAY, H. ‘Kernechtrot’ or Nuclear Fast Red in the Histochemical Detection of Calcareous Corpuscles in Taenia saginata . Nature 176, 701–702 (1955). https://doi.org/10.1038/176701b0

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