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Probable Nature of an Artefact in the Paper Chromatography of Thyroxine caused by Trichloracetic Acid

Nature volume 176, pages 694–695 (1955)Cite this article

Abstract

INTERFERENCE of trichloracetic acid with the paper chromatography of adrenaline analogues and of histamine, using n-butanol/acetic acid/water (4:1:5), has already been reported1,2. A similar artefact is described below, affecting another compound, thyrox-ine, run in a different solvent system, n-butanol/ dioxane/2 N ammonia (4: 1: 5). No effect of this nature is mentioned by Gross3 in his review on the chromatography of thyroid hormones.

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, University of Sheffield, and Sheffield Royal Infirmary,

    J. D. ACLAND

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  1. J. D. ACLAND
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ACLAND, J. Probable Nature of an Artefact in the Paper Chromatography of Thyroxine caused by Trichloracetic Acid. Nature 176, 694–695 (1955). https://doi.org/10.1038/176694a0

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