Abstract
CATION-EXCHANGE resins have proved suitable as adsorbents for chromatographing a number of proteins (see a review by Zittle1). Last year, however, Sober and Peterson2 reported the use of an anion-exchanger as an adsorbent in protein chromatography. These authors used ‘DEAE-Polycel’ (prepared from cellulose) for the chromatographic purification of kidney amidase and some other enzymes. The purpose of the present communication is to describe another system for anion-exchange chromatography of proteins which appears to be useful for a number of rather different proteins.
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References
Zittle, C. A., “Adv. in Enzym.”, 14, 319 (1953).
Sober, H. A., and Peterson, E. A., J. Amer. Chem. Soc., 76, 1711 (1954).
Tiselius, A., Ark. Kemi, 7, 443 (1954).
Gutman, A. B., and Gutman, E. B., J. Clin. Invest., 17, 473 (1938).
Bessey, O. A., Lowry, O. H., and Brock, M. J., J. Biol. Chem., 164, 321 (1946).
Flodin, P., and Porath, J., Biochim. Biophys. Acta, 13, 175 (1954).
Boman, H. G., Biochim. Biophys. Acta, 16, 245 (1955).
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BOMAN, H. Chromatography of Serum and some other Proteins on an Anion-exchange Resin. Nature 175, 898–899 (1955). https://doi.org/10.1038/175898a0
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DOI: https://doi.org/10.1038/175898a0
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