Abstract
PRESENT methods of demonstrating ketosteroids in tissues are generally regarded as being unsatisfactory. 2-Hydroxy-3-naphthoyl hydrazide1 is the reagent most recently introduced for this purpose, yet it condenses only sluggishly with carbonyl groups and fails to distinguish between aldehydes and ketones. A number of phenolic hydrazides have since been tested. Of these, 2 : 4 dihydroxybenzoyl-hydrazide2 is better in that it provides azo-hydrazones of different colours in the ketosteroid group3, but it still lacks adequate condensing reactivity.
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References
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CAMBER, B. Salicyloylhydrazide: a Reagent of Wide Use in Organic and Histochemical Analysis. Nature 174, 1107 (1954). https://doi.org/10.1038/1741107a0
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DOI: https://doi.org/10.1038/1741107a0
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