Abstract
A METHOD for the identification of volatile fatty acids by means of paper chromatography was reported by Hiscox and Berridge1. In this method the acid was applied as the ethylamine salt to the paper, and the chromatogram was run with water as the stationary phase and butanol as the mobile phase in an atmosphere containing ethylamine vapour. In this system the fatty acids exist as anions. On spraying the developed chromatogram with a solution of bromcresol green in ethanol, a blue spot develops where the acid anion resides on the paper.
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References
Hiscox, E. R., and Berridge, N. J., Nature, 166, 522 (1950).
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BURTON, H. Separation of Volatile Acids by Paper Chromatography. Nature 173, 127 (1954). https://doi.org/10.1038/173127a0
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DOI: https://doi.org/10.1038/173127a0
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