Abstract
IT is certainly well known that mammalian enterochromaffin cells acquire a golden-yellow fluorescence after fixation in formalin, as stated by Drs. Shepherd, West and Erspamer1. The views of the last author on their content of enteramine (5-hydroxytryptamine), based on his extensive researches into the enterochromaffin systems of Octopoda and Muricidæ as well as Amphibia and Vertebrata, have not been substantiated histochemically, however, and a considerable body of histochemical opinion holds alternative views. According to Cordier and Lison2, the granules contained an o-diphenol with a short side-chain in the para position; Lison3 maintains this opinion. Gomori4 challenged this view, and concluded that a derivative of resorcinol (m-diphenol) was present. Lillie5, applying his new diazosafranin test, agrees entirely with Gomori.
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References
Shepherd, D. M., West, G. B., and Erspamer, V., Nature, 172, 357 (1953).
Cordier, R., and Lison, L., Bull. Histol. Tech. Micr., 7, 140 (1930).
Lison, L., “Histochimie et cytochimie animales” (Gauthier Villars, Paris, 1953).
Gomori, G., Arch. Path., 45, 48 (1948).
Lillie, R. D., J. Histochem. and Cytochem., 1, 154 (1953).
Erspamer, V., and Boretti, G., Experientia, 6, 348 (1950).
Jacobson, W., Biochem. J., 40, 3 (1946).
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BARTER, R., PEARSE, A. Detection of 5-Hydroxytryptamine in Mammalian Enterochromaffin Cells. Nature 172, 810 (1953). https://doi.org/10.1038/172810a0
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DOI: https://doi.org/10.1038/172810a0
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