Abstract
WHILE usual chromatographic methods have not, in general, proved as useful in the separation of closely related proteins as of low molecular weight substances, chromatography on adsorbents which bind only to the specific reactive sites of biologically active proteins may help to fill that need. I wish to report here the feasibility of elution chromatography of an antibody on its specific adsorbent. The antibody proteins are not only separated from the other constituents of serum, but are, in addition, resolved into components which remain active and distinguishable on repeated chromatography.
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References
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LERMAN, L. Antibody Chromatography on an Immunologically Specific Adsorbent. Nature 172, 635–636 (1953). https://doi.org/10.1038/172635a0
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DOI: https://doi.org/10.1038/172635a0
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