Origin and Fate of the Virus Bundles in Nuclear Polyhedroses

Abstract

FOR a close study of the intranuclear cytology of sections of lepidopterous larvæ infected with nuclear polyhedral viruses, it is necessary, in addition to methods for staining the polyhedra¹, to have some method for staining the virus bundles which electron microscopy shows are embedded within the polyhedra. It has been found that this can be done readily, and the origin and fate of the virus bundles can then be traced. Owing to the limitations of ordinary light microscopy, the term virus bundle must perforce be used here to include not only bundles of mature virus rods, but also the bodies of a similar character which are liable to become embedded within polyhedra and within which virus rods may differentiate.