Abstract
DURING the past few years, differential centrifugation of tissue homogenates has become one of the major techniques of cytology and biochemistry alike. Most of those who have applied this new technique describe the conditions of centrifugation by mentioning only the duration of operation and the centrifugal field prevailing in the middle (or at the bottom) of the tube; the type of centrifuge used is sometimes also mentioned. However, the information thus supplied is not sufficient to enable other workers to reproduce the conditions, especially when another type of instrument is to be used. This fact may be partly responsible for the wide variations which are sometimes encountered between results obtained in different laboratories.
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Pickels, E. G., J. Gen. Physiol., 26, 341 (1943).
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DE DUVE, C., BERTHET, J. Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation. Nature 172, 1142 (1953). https://doi.org/10.1038/1721142a0
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DOI: https://doi.org/10.1038/1721142a0
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