Abstract
A MAJOR difficulty in the estimation of urinary corticosteroids arises from their destruction under conditions necessary for the complete hydrolysis of steroid conjugates. Extraction of corticosteroids from non-hydrolysed or partly hydrolysed urine yields only a small fraction of the amount released by enzymatic hydrolysis with β-glucuronidase1. The existing procedures have been authoritatively discussed and their inadequacy pointed out by Marrian2.
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References
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Marrian, G. F., J. Endocrinol., 7, lxix (1951).
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NORYMBERSKI, J. Determination of Urinary Corticosteroids. Nature 170, 1074–1075 (1952). https://doi.org/10.1038/1701074b0
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DOI: https://doi.org/10.1038/1701074b0
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