Abstract
THE importance of trichloracetic acid as an agent for the removal of nucleic acids from their combination in nucleoproteins has been amply demonstrated by Schneider1. The method is now widely used by various authors in different cytochemical procedures. In order to find out whether any differential reactivity of the chromosome parts—namely, euchromatin and heterochromatin—with fuchsin sulphurous acid appears after such treatment, experiments were carried out, mainly on root-tips of Allium cepa and Hordeum vulgare. A series of different concentrations of trichloracetic acid was prepared and the root-tips treated under different conditions.
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References
Schneider, W. C., J. Biol. Chem., 161, 293 (1945).
Levan, A., Hereditas, 32, 449 (1946).
Feulgen, R., and Rossenbeck, H., Z. physiol. Chem., 135, 203 (1924).
Overend, W. G., and Stacey, M., Nature, 163, 538 (1949).
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SHARMA, A. Trichloracetic Acid and Feulgen Staining. Nature 167, 441–442 (1951). https://doi.org/10.1038/167441b0
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DOI: https://doi.org/10.1038/167441b0
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