Abstract
UNTIL quite recently, the substances used for supporting the stationary phase in partition chromatography have held the more polar solvent preferentially. Chromatograms of this type have been very satisfactory for the separation of substances with predominantly water-soluble character; but several workers have pointed out that full advantage cannot be taken of small differences in partition coefficients if these are in the range which gives R values1 much above 0.5. In the case of compounds such as the higher fatty acids, where the partition coefficients greatly favour the non-aqueous phase, these ideal conditions cannot be realized, and it has been suggested that a system in which the phases are reversed would lead to useful results. Attempts have been made by Boscott2, using cellulose acetate, and by Boldingh3, using rubber powder, to hold the less polar phase on the inert support, and recently Howard and Martin4 have described the use of kieselguhr, impregnated with silane, for a reversed-phase partition column.
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References
Martin, A. J. P., and Synge, R. L. M., Biochem. J., 35, 1358 (1941).
Boscott, R. J., Nature, 159, 342 (1947).
Boldingh, J., Discussions of the Faraday Society No. 7, “Chromatographic Analysis”, 162 (1949).
Howard, G. A., and Martin, A. J. P., Biochem. J., 46, 532 (1950).
Sanger, F., Biochem. J., 39, 507 (1945).
Partridge, S. M., and Davis, H. F., Biochem. J., 46, 532 (1950).
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PARTRIDGE, S., SWAIN, T. A Reversed-Phase Partition Chromatogram using Chlorinated Rubber. Nature 166, 272–273 (1950). https://doi.org/10.1038/166272b0
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DOI: https://doi.org/10.1038/166272b0
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