Abstract
WE have studied a strain of Chætomium globosum which was cultured on the basal medium of Buston and Basu1 (NaNO3, 2 gm.; KH2PO4, 1 gm.; KCl, 0.5 gm.; MgSO4.7H2O, 0.5 gm. ; FeSO4, 0.01 gm., in 1,000 ml. water) with the addition of 5 per cent sucrose and 2 per cent yeast extract. 50-ml. portions contained in 500-ml. flasks were inoculated with 1 ml. of a spore suspension and incubated at 25°. Samples were withdrawn aseptically from the culture flasks at intervals and spotted on a paper chromatogram2 and developed with n-butanol – water for 56 hr.
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References
Buston and Basu, J. Gen. Microbiol., 2, 162 (1948).
Forsyth and Webley, Nature, 162, 150 (1948).
Forsyth, Nature, 161, 239 (1948).
Flood, Hirst and Jones, J. Chem. Soc., 1679 (1948).
Nelson, J. Biol. Chem., 153, 375 (1944).
Forsyth and Webley, J. Gen. Microbiol. (in the press).
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DUFF, R., EASTWOOD, D. Use of the Arsenomolybdate–Somogyi Reagent in Quantitative Paper Chromatography, and its Application to the Study of Sucrose Utilization by a Fungus. Nature 165, 848–849 (1950). https://doi.org/10.1038/165848b0
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DOI: https://doi.org/10.1038/165848b0
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