Abstract
THE difficulty which is usually met with in counter-staining smears and sections stained with Crystal Violet can be largely overcome by the use of ‘Cellosolve’ (ethylene glycol monoethyl ether) as a dehydrating agent. Crystal and Gentian Violet are considerably less soluble in ‘Cellosolve’ than alcohol and hence are not removed quite so rapidly from the cells. Provided that the cells are fixed sufficiently, the shrinkage caused by this fluid is negligible.
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SHAW, G. A Rapid Permanent Method for Chromosomes. Nature 165, 408 (1950). https://doi.org/10.1038/165408a0
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DOI: https://doi.org/10.1038/165408a0
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