Abstract
THE identification of the free amino-groups of proteins by the method introduced by Sanger1 is now widely practised, its most attractive feature being the resistance of most dinitrophenyl amino-acids to acid hydrolysis. There are, however, two difficulties in the application of this technique : first, several systems of solvents are used, some of which do not dissolve the dinitrophenyl compounds very well. Secondly, chromatographic resolution of some of the derivatives is poor, the masking of the polar amino-group by the massive dinitrophenyl residue being generally disadvantageous.
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Biochem. J., 39, 507 (1945).
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MILLS, G. Identification of Dinitrophenylamino-Acids. Nature 165, 403 (1950). https://doi.org/10.1038/165403a0
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DOI: https://doi.org/10.1038/165403a0
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