Abstract
Two requirements for the successful preparation of high-resolution autoradiographs are the intimate contact between section and emulsion, and an emulsion-layer which is thin and of sufficiently fine grain. The stripping-emulsion technique was developed by Pelc1 to meet these requirements, and was successfully applied to the location of iodine-131 in rat thyroid. In this technique, section and emulsion are brought into intimate contact under water. Thus it precludes the location of water-soluble tracers; moreover, the use of conventional fixing and dehydrating agents can seriously affect the concentration and distribution of water-soluble tracer in the tissue.
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References
Pelc, S. R., Nature, 160, 749 (1947).
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WINTERINGHAM, F., HARRISON, A. & HAMMOND, J. Autoradiography of Water-Soluble Tracers in Histological Sections. Nature 165, 149–150 (1950). https://doi.org/10.1038/165149b0
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DOI: https://doi.org/10.1038/165149b0
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