Abstract
THE blackening by osmium tetroxide of the perinuclear layer in various animal cells is well known (see, for example, Hirschler1). Recently, with an electron microscope, Gessler and Fullam2 investigated guinea pig liver fixed with osmium tetroxide and cut with the high-speed microtome; they observed that the ‘nuclear membrane' is sharply shown, and that, generally, the fat-containing parts of the tissue appear darker in these electron photomicrographs.
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References
Hirschler, J., C.R. Soc. Biol., 93, 663 (1925).
Gessler, A. E., and Fullam, E. F., Amer. J. Anat., 78, 245 (1946).
Baud, C. A., C.R. Acad. Sci., 224, 1122 (1947).
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BAUD, C. A Cytochemical Study of the Perinuclear Lipidic Layer in the Liver Cell. Nature 161, 559 (1948). https://doi.org/10.1038/161559a0
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DOI: https://doi.org/10.1038/161559a0
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