Abstract
PARTITION chromatography on paper1 has not hitherto been reported for the analysis of riboflavine, its nucleotides and derivatives. However, ‘Collidine', and a butyl alcohol-acetic acid mixture (shake 4 parts butyl alcohol with 1 part glacial acetic acid and 5 parts water, and use the upper layer), are two very suitable solvents for separating various flavine compounds, identified by their characteristic green fluorescence in ultra-violet light, which will detect as little as 0·01 µgm. flavine on the paper. The separated substances can be easily and quantitatively removed from the paper by the method of Dent2 and used for enzymic or other experiments.
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References
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Dent, C. E., Biochem. J., 41, 245 (1947).
Comline, R. S., and Whatley, F. R., see following communication.
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CBAMMER, J. Paper Chromatography of Flavine Nucleotides. Nature 161, 349–350 (1948). https://doi.org/10.1038/161349c0
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DOI: https://doi.org/10.1038/161349c0
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