Abstract
THE successful use of desiccated 9–11-day chick embryo as a source of growth-promoting factor was reported in 1940 (Peacock and Shukoff). Since then, repeated tests have been made with desiccates stored for periods up to 2½ years at 4°C. All the samples tested were equally effective and retained their growth-promoting potency and their capacity to coagulate plasma. Apart from the advantages of essential economy in war-time, the use of dried embryo greatly simplifies the technique of tissue culture and helps to standardize results, as it tends to eliminate any individual differences between embryos, and one batch of desiccate can be used for a long series of experiments. Freezing at 0°C. does not appear to affect the desiccates, but storage in a refrigerator at 4°C. has proved quite satisfactory.
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SHUKOFF, R. Use of Desiccated Chick Embryo in Tissue Culture Technique. Nature 150, 461 (1942). https://doi.org/10.1038/150461b0
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DOI: https://doi.org/10.1038/150461b0
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