Abstract
THE variability in the responses of a group of animals to the administration of the same substance usually necessitates taking, as criterion of response, the average for the group. In many instances, even with the use of a large number of animals, the average response shows significant variations when the administration of the same dose is repeated on different occasions. If the assay is to have any quantitative value, it is essential to use a standard of reference, with which the substance under test can be directly compared. Thus the individual variations within the group are controlled by the use of a sufficient number of animals, such that each time the group selected is truly representative of the population available for test, whilst the variations in sensitiveness from day to day are controlled by the use of the standard of reference.
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References
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Quantitative Methods in Vitamin Assays. Nature 129, 514–515 (1932). https://doi.org/10.1038/129514a0
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DOI: https://doi.org/10.1038/129514a0
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