Abstract
The Notch1 gene was previously found to be targetted by provirus insertion in a high proportion of T-cell lymphomas arising in Moloney MuLV-inoculated MMTVD/myc transgenic mice. Proviral activation of Notch1 was associated with overexpression of truncated Notch1, deleted of the sequences coding for the extracellular domain. The high levels of truncated Notch1 RNA and proteins in these tumors are thought to be involved in the oncogenic transformation. However, in addition to these truncated RNA and proteins, high level expression of full-length Notch1 RNA and proteins was also observed in several tumors, suggesting that they could also contribute to the transformation process. To test this hypothesis, we used a genetic approach and studied MMTVD/myc transgenic mice in which one of the Notch1 alleles was mutated by targeted mutagenesis (Notch1+/− mice). Heterozygote (Notch1+/−) and wild-type (Notch1+/+) transgenic mice were inoculated with Moloney MuLV and the frequency of Notch1 rearrangements was compared between both groups. Notch1 was rearranged at similar frequencies in both groups, indicating that the full-length Notch1 allele is dispensable in tumors harboring an activated Notch1 allele.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Girard, L., Jolicoeur, P. A full-length Notch1 allele is dispensable for transformation associated with a provirally activated truncated Notch1 allele in Moloney MuLV-infected MMTVD/myc transgenic mice. Oncogene 16, 517–522 (1998). https://doi.org/10.1038/sj.onc.1201562
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1201562
Keywords
This article is cited by
-
High-throughput insertional mutagenesis screens in mice to identify oncogenic networks
Nature Reviews Cancer (2009)
-
What retroviruses teach us about the involvement of c-Myc in leukemias and lymphomas
Leukemia (2002)
-
Evidence for the transforming activity of a truncated Int6 gene, in vitro
Oncogene (2001)