Abstract
IT is known that it is very difficult to obtain well-preserved human material. Few medical men realise that five or ten minutes after the tissue has been removed, or after death, plasmolytic changes supervene, and in the fixed and stained sections the chromosomes have clumped badly, and the delicate lipoid cytoplasmic organellæ have become abnormal, or completely macerated. Recently, I have been studying certain human material, and find that nearly every type of histological preparation can be made from two fixing fluids as follows: one of the surgeon's assistants is given two bottles, one of Da Fano's cobalt nitrate formalin fluid, and one of Regaud's formalin-bichromate fluid. Pieces of tissue as large as the thumb may be thrown into these bottles, and afterwards cut into smaller pieces when they have been brought to the laboratory. It is better to change into new fluid at once, especially if the organ is very vascular, and the fixing fluid mixed with blood.
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GATENBY, J. Fixation of Human Embryological and Cytological Material. Nature 112, 830 (1923). https://doi.org/10.1038/112830a0
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DOI: https://doi.org/10.1038/112830a0
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