Natural ribozymes can cleave RNA and single-stranded DNA (ssDNA) by transesterification or a blend of hydrolytic and transesterification reactions. Now, ribozymes have been discovered that catalyze the hydrolytic cleavage of ssDNA. Similar ribozymes could potentially replace large, immunogenic, protein-based nucleases in gene therapies.
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King, M.B., Lapinaite, A. RNA-based programmable DNA cleavage. Nat Chem Biol 20, 664–665 (2024). https://doi.org/10.1038/s41589-024-01625-1
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DOI: https://doi.org/10.1038/s41589-024-01625-1