Imaging the immune system articles within Nature Communications

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  • Article
    | Open Access

    CD8 T cells can protect the liver from viral infection, but can also result in severe liver damage and organ failure. Here, the authors develop a mouse model reflecting fulminant CD8 T cell mediated viral hepatitis, which occurs in a perforin-dependent manner that is protected by the use of perforin inhibitors.

    • M. Welz
    • , S. Eickhoff
    •  & W. Kastenmüller

  • Article
    | Open Access

    Microvilli can participate in adhesion or migration of T cells, but whether they are involved in function regulation is unclear. Here the authors show that T cell microvilli form budding vesicles containing T cell signalling components for deposition onto antigen presenting cells (APC) and modulation of APC functions.

    • Hye-Ran Kim
    • , YeVin Mun
    •  & Chang-Duk Jun

  • Article
    | Open Access

    Memory B cells need to be reactivated to produce high affinity antibody responses on subsequent antigen encounters. Here the authors show that memory B cells localise to lymph node subcapsular proliferative foci (SPF), which have distinct properties from the germinal centre, for rapid expansion and the induction of B memory responses.

    • Imogen Moran
    • , Akira Nguyen
    •  & Tri Giang Phan

  • Article
    | Open Access

    Endocytosis of T cell receptors (TCR) and their polarized recycling back to the plasma membrane is crucial for T cell activation; however, the underlying mechanisms remain unclear. Here the authors follow TCR and show that a mobile endocytic network connects clathrin-independent receptor endocytosis to recycling which is required for T cell activation.

    • Ewoud B. Compeer
    • , Felix Kraus
    •  & Jérémie Rossy

  • Article
    | Open Access

    T cell activation is critically controlled by T cell receptor (TCR) signalling. Here the authors show, using live cell imaging, atomic force microscopy and modelling simulation, a prompt separation of TCR and CD45 that negatively correlates with TCR activation, supporting a refined kinetic segregation model of TCR signalling.

    • Yair Razvag
    • , Yair Neve-Oz
    •  & Eilon Sherman

  • Article
    | Open Access

    Monocytes constitutively adhere and crawl along the glomerular endothelium and are thought to contribute to glomerulonephritis. Here the authors use multiphoton microscopy to show local antigen presentation by MHCII+ monocytes to T cells in glomerular capillaries of mice.

    • Clare L. V. Westhorpe
    • , M. Ursula Norman
    •  & Michael J. Hickey

  • Article
    | Open Access

    Tissue-resident macrophages are derived from yolk sac progenitors but how and when these progenitors enter is unclear. Here the authors use fate mapping and intravital microscopy to track the movement of resident macrophage precursors from the yolk sac to fetal tissues during development.

    • C. Stremmel
    • , R. Schuchert
    •  & C. Schulz

  • Article
    | Open Access

    Longitudinal imaging of bone marrow would shed insight into long-term cellular dynamics within this compartment. Here, the authors develop a multi-photon imaging approach for the mouse femur and reveal extensive vascular plasticity within the bone marrow during bone healing and steady-state homeostasis.

    • David Reismann
    • , Jonathan Stefanowski
    •  & Raluca A. Niesner

  • Article
    | Open Access

    The ability to quantify the organization of cell membrane molecules is limited by the density of labeling and experimental conditions. Here, the authors use super-resolution optical fluctuation (SOFI) for molecular density and clustering analyses, and investigate nanoscale distribution of CD4 glycoprotein.

    • Tomáš Lukeš
    • , Daniela Glatzová
    •  & Marek Cebecauer

  • Article
    | Open Access

    ROCK is associated with T cell movement in lymph nodes. Here the authors use an LPS lung damage model and two-photon imaging to show that CD8+ T cells in lung tissue engage in ROCK-dependent fast linear migration alternating with bursts of slower confined migration that together optimize contact with target cells.

    • Paulus Mrass
    • , Sreenivasa Rao Oruganti
    •  & Judy L. Cannon

  • Article
    | Open Access

    The growth of lymph nodes in response to infection requires lymphangiogenesis. Dubey et al. show that the mesenteric lymph node lymphangiogenesis upon helminth infection depends on the signaling loop between the B and fibroblastic reticular cells (FRCs), whereby the FRCs respond to lymphotoxin secreted by B cells by releasing B cell activating factor.

    • Lalit Kumar Dubey
    • , Praneeth Karempudi
    •  & Nicola L. Harris

  • Article
    | Open Access

    There are few methods available that can quantify relationships between cell types in tissue images. Here the authors present a quantitative method to evaluate cellular organization, validated in the mouse thymus and spinal cord, called Multitaper Circularly Averaged Spectral Analysis (MiCASA).

    • Andrew Sornborger
    • , Jie Li
    •  & Nancy R. Manley

  • Article
    | Open Access

    Characterization of T cell antigen specificity human blood is challenging due to the low clonal frequencies. Here the authors develop a fluorescent microscopy-based method to detect antigen-specific CD8 T cell activation, and apply it to characterize the anti-CMV repertoire.

    • Nadia Anikeeva
    • , Dolores Grosso
    •  & Yuri Sykulev

  • Article
    | Open Access

    Sarcolemma lesions are sealed by a repair patch of lipids and proteins that prevents cell death and myopathy. Here the authors show that the "eat-me" signal phosphatidylserine is sorted from adjacent sarcolemma to the repair patch in a Dysferlin dependent process in zebrafish and human cells.

    • Volker Middel
    • , Lu Zhou
    •  & Uwe Strähle

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