Fluorescence resonance energy transfer articles within Nature Communications

Featured

  • Article
    | Open Access

    Here, using single molecule FRET, the unfolding and folding of a discontinuous two-domain protein was studied. The authors find that a dynamic, intermediate population entropically limits the rate of folding while the order of domain folding is kept in a slow-folding mutant.

    • Ganesh Agam
    • , Anders Barth
    •  & Don C. Lamb

  • Article
    | Open Access

    The precise role of cochaperones and ATP hydrolysis in driving Hsp90’s chaperone cycle is largely unclear. Here, the authors use single-molecule FRET to show that several cochaperones are necessary to establish directionality in Hsp90’s conformational cycle.

    • Leonie Vollmar
    • , Julia Schimpf
    •  & Thorsten Hugel

  • Article
    | Open Access

    In this work, the authors demonstrate the application of multi-parameter photon-by-photon hidden Markov modeling (mpH2MM) on alternating laser excitation (ALEX)-based smFRET measurements. The utility of mpH2MM in identifying and quantifying dynamic biomolecular sub-populations is demonstrated in three different systems.

    • Paul David Harris
    • , Alessandra Narducci
    •  & Eitan Lerner

  • Article
    | Open Access

    Sea urchin hyperpolarization-activated cyclic nucleotide-gated (spHCN) ion channels channels are activated by membrane hyperpolarization instead of depolarization and undergo inactivation with hyperpolarization. Here authors apply transition metal ion FRET, patch-clamp fluorometry and Rosetta modeling to measure differences in the structural rearrangements between activation and inactivation of spHCN channels.

    • Gucan Dai
    • , Teresa K. Aman
    •  & William N. Zagotta

  • Article
    | Open Access

    The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.

    • Danai Laskaratou
    • , Guillermo Solís Fernández
    •  & Hideaki Mizuno

  • Article
    | Open Access

    Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.

    • Benjamin Ambrose
    • , James M. Baxter
    •  & Timothy D. Craggs

  • Article
    | Open Access

    Single-molecule sensing is very challenging due to weak emitted signals and environmental interference. Here the authors design a method (i-SET) for single molecule sensing with core-shell upconverting nanoparticles, which relies on signal enhancement by the activator-rich probes to quantify fluorophores attached to a single nanoparticle.

    • Jian Zhou
    • , Changyu Li
    •  & Renren Deng

  • Article
    | Open Access

    Genetically encoded voltage indicators (GEVIs) allow visualisation of fast action potentials in neurons but most are bright at rest and dimmer during an action potential. Here, the authors engineer electrochromic FRET GEVIs with fast, bright and positive-going fluorescence signals for in vivo imaging.

    • Ahmed S. Abdelfattah
    • , Rosario Valenti
    •  & Eric R. Schreiter

  • Article
    | Open Access

    TagRFP is a bright red fluorescent protein, but undergoes photoconversion to a dark state, making it undesirable for conventional fluorescence microscopy. Here, the authors introduce stabilising mutations to create super-TagRFP (stagRFP) and demonstrate its application as both a FRET acceptor and FRET donor.

    • Gary C. H. Mo
    • , Clara Posner
    •  & Jin Zhang

  • Article
    | Open Access

    A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation.

    • Rebecca J. Wood
    • , Angelique R. Ormsby
    •  & Danny M. Hatters

  • Article
    | Open Access

    Chromatin fibers undergo continuous structural rearrangements but their dynamic architecture is poorly understood. Here, the authors use single-molecule FRET to determine the structural states and interconversion kinetics of chromatin fibers, monitoring their effector protein-dependent dynamic motions.

    • Sinan Kilic
    • , Suren Felekyan
    •  & Beat Fierz

  • Article
    | Open Access

    Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.

    • Manu Ben-Johny
    • , Daniel N. Yue
    •  & David T. Yue

  • Article
    | Open Access

    Activation-induced deoxycytidine deaminase (AID) induces somatic hypermutation and class-switch recombination during transcription of immunoglobulin genes. Here the authors use single-molecule FRET to show that AID translocates together with RNA polymerase and scans within stalled transcription bubbles.

    • Gayan Senavirathne
    • , Jeffrey G. Bertram
    •  & David Rueda

  • Article |

    Photochromic Förster resonance energy transfer endows nanosensors with photoswitchable fluorescence properties. Diaz et al. present a system with two photostationary end states, one of which exhibits constant quenching of the quantum dot donor independent of its mean distance to the photochromic acceptors.

    • Sebastián A. Díaz
    • , Florencia Gillanders
    •  & Thomas M. Jovin

  • Article
    | Open Access

    DNA is a useful molecule with which to construct nanomaterials with controllable functionalities. Here, the authors fabricate photonic wires by appending dye molecules at set positions along DNA structures, and show how FRET performance can be tuned by modifying dye separation.

    • Susan Buckhout-White
    • , Christopher M Spillmann
    •  & Igor L. Medintz

  • Article |

    Genetically encoded voltage sensors are useful tools for the analysis of membrane potential and its influence on cell function. Here, the authors present a range of these sensors with varying colours for rapid and sensitive neuronal voltage imaging.

    • Peng Zou
    • , Yongxin Zhao
    •  & Adam E. Cohen

  • Article |

    The activity of heat shock proteins (Hsp) is modified by binding to cochaperones. Here, the authors develop a four-colour FRET system to show that cochaperone p23 binding to Hsp90 strengthens the ATP-dependent directionality, thus validating their approach for the study of other multicomponent protein machines.

    • C. Ratzke
    • , B. Hellenkamp
    •  & T. Hugel

  • Article |

    Genetically encoded optical voltage sensors measure the electrical activity of various tissues with limited effectiveness, due to the sensors’ suboptimal performance metrics. Gong et al.create a sensor with increased brightness, fast kinetics and improved dynamic ranges when compared with previous sensors.

    • Yiyang Gong
    • , Mark J. Wagner
    •  & Mark J. Schnitzer

  • Article |

    Förster resonance energy transfer, where energy is transferred between luminescent states, is a mechanism used for applications in photovoltaics or bio-imaging. Here, the authors show that these energy transfer rates are independent of the photonic environment, providing valuable feedback for applications.

    • Freddy T. Rabouw
    • , Stephan A. den Hartog
    •  & Andries Meijerink

Browse broader subjects

Browse Fluorescence resonance energy transfer across other nature.com journals