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| Open AccessEfficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
Plant genome editing typically relies upon transgenic intermediates, which is a concern given the current regulatory requirements concerning GMOs. Here, Zhang et al. describe a method to edit wheat genomes by transiently expressing CRISPR/Cas9 DNA or RNA, and are able to generate mutant plants with no detectable transgenes.
- Yi Zhang
- , Zhen Liang
- & Caixia Gao
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Article
| Open AccessImmunogenomic engineering of a plug-and-(dis)play hybridoma platform
Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.
- Mark Pogson
- , Cristina Parola
- & Sai T. Reddy
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Article
| Open AccessWriting of H3K4Me3 overcomes epigenetic silencing in a sustained but context-dependent manner
Epigenome editing by zinc finger (ZF) and CRISPR-dCas9 technologies can induce or repress gene expression. Here, the authors show that histone methyltransferase PRDM9 fused to either dCas9 or ZF proteins can sustain gene re-expression, and H3K79me is required for stable gene re-expression.
- David Cano-Rodriguez
- , Rutger A F. Gjaltema
- & Marianne G Rots
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Article
| Open AccessCRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.
- Jonathan L. Schmid-Burgk
- , Klara Höning
- & Veit Hornung