Volume 15 Issue 6, June 2020

The authors demonstrate two variants of the cellular thermal shift assay (CETSA) protocol—the melt curve and the isothermal dose–response—for use on intact cell or lysate P. falciparum samples for identification of antimalarial drug targets.

This protocol combines two genome-wide technologies, DamID and CEL-Seq2, to simultaneously profile DNA interactions with a POI and transcriptomes in single cells.

Global Natural Product Social Molecular Networking (GNPS) is an online tandem mass spectrometry (MS²) data curation and analysis infrastructure. This protocol describes how to use GNPS to explore uploaded metabolomics data.

This protocol describes how to construct and use an open-source robot for automatically performing cranial microsurgeries such as skull thinning and various-sized craniotomies in mice.

Ice formation has hindered organ preservation below 0 °C. In this protocol, human livers are preconditioned with cryoprotective agents during machine perfusion and then supercooled to avoid ice formation.

High-resolution structural analysis of macromolecular complexes by cryo-ET requires extremely thin samples. This protocol describes how to prepare thin specimens using FIB milling from frozen cells on grids, which enables direct structural analysis of biomolecules in their native environments, i.e., cells.

This protocol details the assembly and use of the wide field-of-view nematode tracking platform (WF-NTP), which enables the simultaneous analysis of thousands of worms with respect to multiple behavioral parameters.

This protocol describes how to characterize the 3D topology of long noncoding RNAs. The authors provide detailed step-by-step procedures for the complete workflow from lncRNA isolation to AFM imaging and SAXS analysis.