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This protocol describes procedures for the high-throughput in situ X-ray screening of and data collection from soluble and membrane-protein crystals at room temperature and under cryogenic conditions using Mylar-based film sandwich plates.
In this protocol, mouse or human colorectal cancer organoids are transplanted into the cecal epithelium. The transplanted organoids grow into a single primary tumor mass within the intestinal tract and tumor cells are capable of metastasis.