Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
The particle-swarm optimization–enhanced thromboSeq pipeline enables RNA sequencing and cancer classification from blood platelet RNA, using self-learning and swarm intelligence–enhanced bioinformatics algorithms.
Brodkorb et al. provide a standardized static in vitro protocol for the study of gastrointestinal food digestion and the analysis of digestion products.
This protocol describes how to measure nanoscale diffusion dynamics of proteins and lipids in cell membranes using STED–FCS. The protocol contains procedures for calibration, performing point and scanning STED–FCS measurements, and data analysis.
The human cochlea is a rich source of endogenous DNA. This protocol describes how to use a sandblaster to isolate the cochlea from ancient skeletal remains and prepare bone powder for downstream DNA extraction.
Zhang and Lyden describe a protocol for asymmetric-flow field-flow fractionation (AF4) to separate and characterize extracellular nanoparticles for investigation of their biogenesis, function and potential in molecular diagnostics and therapeutics.
This protocol describes how to use a microfluidics system to produce human induced pluripotent stem cells at high efficiency and low cost using a small starting cell number.
Sonoporation is a minimally invasive gene delivery method mediated by ultrasound. This protocol describes step-by-step procedures for sonoporation in pigs and mice.
This protocol describes procedures for isolating and purifying uncondensed formaldehyde- and propionaldehyde-stabilized lignins from lignocellulosic biomass, and for recovering highly digestible cellulose and stabilized xylose.
This protocol describes an approach for super-resolution imaging by 10× physical expansion of the samples (X10 microscopy). In addition, the authors provide guidelines for determining the expansion and distortion factors.
In this protocol, two mice meet in a tube and the more dominant one pushes out the other one. The social hierarchy of a group of mice can thus be measured.
Here the authors describe CAPTURE, a versatile and sensitive protocol to detect spacer-acquisition events in native CRISPR arrays using nested PCR amplification and amplicon size selection.
Here, the authors provide protocols for CRISPR–Cas9-based genetic manipulation of Candida albicans, using a gene-drive strategy that allows genetic interaction networks to be established for this important fungal pathogen.
This protocol describes the proximity ligation assay for RNA (PLAYR). PLAYR can be used to simultaneously detect at least 27 RNA transcripts using flow or mass cytometry and can be combined with protein detection via conventional antibody staining.
MAGeCKFlute is an algorithm for the analysis and visualization of CRISPR screen data. Starting from sequencing reads and an sgRNA library, the algorithm normalizes results and represents them as pathway enrichment classifications.
This protocol describes a platform that integrates mammalian lncRNAs and whole genomes with the LongTarget program for genome-wide lncRNA-binding prediction. A choice of four pipelines allows searches to be tailored to different biological questions.
This protocol describes how to fabricate, calibrate, and use micropipette force sensors for measurements in the sub-nanonewton to millinewton range. The micropipettes can be used on samples ranging from single cells to millimeter-sized organisms.
High-silica zeolites are widely used catalysts; those with different structural topologies are used for different applications. This protocol describes the assembly–disassembly–organization–reassembly (ADOR) process for preparing new zeolites.
A small laparotomy is carried out, and human cancer cells are implanted into the mouse bladder lumen. This reproduces the pathobiological events of human bladder cancer invasion in mice.
This protocol describes enhanced number and brightness (eN&B), an approach that uses fluorescence fluctuation spectroscopy data to directly measure the oligomerization state and dynamics of fluorescently tagged proteins in living cells.