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This protocol describes the fabrication of AngioChip, a microfabricated scaffold for engineering vascularized tissues. In addition, the protocol contains procedures for immunostaining, drug screening and surgical anastomosis of the AngioChip.
This protocol describes a noninvasive approach to evoke microglial engulfment and reduce amyloid levels in mouse brain. The authors describe assembly and operation of a light-flicker system, as well as assessment of the molecular and cellular effects.
1,2-diamines are commonly found in pharmaceutically relevant compounds. They can be prepared from diazides. This protocol describes an electrocatalytic method for preparing a wide range of diazides from alkenes, using a manganese catalyst.
This protocol describes how to combine expansion microscopy (ExM) with structured illumination microscopy (SIM). ExM–SIM is exemplified by super-resolution analysis of the synaptonemal complex (SC) and single-particle averaging of SC proteins.
This protocol describes a pig model in which lungs are transplanted into recipients who are subsequently monitored for 3 d after surgery to study ischemia reperfusion injury and early graft function.
This protocol describes a proteomics approach for quantifying newly synthesized proteins (NSPs). NSPs are pulse-labeled with the methionine analog AHA or a heavy-isotope version of it, followed by AHA-peptide enrichment and LC-MS/MS analysis.
In this behavioral task, two objects are placed at specific locations at different times, and the response of mice and rats to these positions is assessed. The results indicate the rodents’ ability to recognize changes in spatial pattern separation.
HyperTRIBE uses a hyperactive RNA-editing enzyme fused to an RNA-binding protein (RBP) to mark the target RNA transcripts of the RBP by converting adenosine to inosine near the binding sites with increased efficiency and reduced sequence bias.
Ingber and colleagues describe how to generate kidney glomerular podocytes from human pluripotent stem cells and how to engineer a human kidney Glomerulus Chip.
This protocol describes the design of DNA origami-based shape IDs and their applications in the identification of single-nucleotide polymorphisms using atomic force microscopy.
This protocol describes how to carry out the sucrose preference test (SPT) in a standardized way to reduce data variability. The SPT is a widely used behavioral assay to monitor depression-related behavior in rodents.
This protocol describes procedures for evoking and tracking zebrafish eye movement, integrating optogenetics and calcium imaging, and analyzing optokinetic data using the customizable, LabVIEW-based software ZebEyeTrack.
This protocol describes a workflow for multiplexed deep-scale, quantitative proteome and phosphoproteome analysis of tumor tissue samples. The procedure includes step-by-step instructions for all stages, from sample preparation to data analysis.
This protocol describes how to direct the assembly of extra-embryonic trophoblast stem cells and embryonic stem cells to recapitulate post-implantation mouse embryogenesis.
This protocol describes how to computationally process, integrate, visualize, and analyze anatomical and functional data obtained during intracranial electroencephalography (iEEG) of the human brain.
The wettability of a surface can be determined by measurement of the contact angle between a water droplet and the surface. This protocol describes sessile-drop goniometry for the measurement of advancing and receding contact angles.
This protocol describes how to build an optical time-stretch microscope and a cell-focusing microfluidic device for high-throughput (>10,000 cells per s) single-cell imaging by optofluidic time-stretch microscopy.
Nanozymes are nanomaterials that possess intrinsic enzyme-like properties and are increasingly used in various biological applications. This protocol describes standardized assays of the catalytic activity and kinetics of peroxidase-like nanozymes.
Xiao et al. present an approach combining hydrogen–deuterium-exchange mass spectrometry (HDXMS), cross-linking MS (CXMS), and disulfide trapping to elucidate the architecture of protein complexes, using the β2AR-β-arrestin1 complex as an example.
A series of diverse in vivo imaging protocols for evaluating hemodynamic response and tissue oxygenation in mouse models of neurological and vascular disorders are described.