Volume 20 Issue 4, April 2023

It takes juggling expertise to be an academic and a parent.

High-resolution sequencing methods that capture the epigenetic landscape within the T cell receptor (TCR) gene loci are pivotal for a fundamental understanding of the epigenetic regulatory mechanisms of the TCR repertoire. In our opinion, filling the gaps in our understanding of the epigenetic mechanisms regulating the TCR repertoire will benefit the development of strategies that can modulate the TCR repertoire composition by leveraging the dynamic nature of epigenetic modifications.

The tumor microenvironment is a source of cancer’s strength. Multi-omics and new spatial techniques stand to find ways to weaken it.

Outbreak.info empowers real-time variant monitoring and tracing of associated publications and resources during the ‘infodemic’ of SARS-CoV-2.

Although structural variation is less explored than single-nucleotide variation, recent studies have shown it to be associated with several human diseases. Three fresh computational methods might help to elucidate this inadequately understood part of our genetic makeup.

A self-inactivating rabies virus tracer allows long-term monitoring and transcriptomic profiling of synaptically connected cells.

New three-photon miniature microscopes open the study of neuronal networks to those deep in the brains of behaving animals.

We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.

This Review describes advances in cryogenic electron tomography on focused ion beam lamellae, highlighting the key benefits of this technology for in situ structural biology and discussing important future directions.

The outbreak.info genomic reports provides comprehensive and detailed information about SARS-CoV-2 lineages and mutations worldwide, which facilitates near real-time genomic surveillance.

An international blind study confirms that smFRET measurements on dynamic proteins are highly reproducible across instruments, analysis procedures and timescales, further highlighting the promise of smFRET for dynamic structural biology.

Outbreak.info Research Library collects and integrates COVID-19 information from various repositories, enabling one-stop search for publications, clinical trials, datasets and other resources.

Two mScarlet variants with high brightness and fast maturation times have been evolved. These variants behave favorably as fusion tags and Förster resonance energy transfer acceptors.

Two miniature microscopes with innovative light paths are described and applied to imaging of juvenile zebra finches and mice.

SVDSS combines multiple strategies to improve detection of structural variation using low-error long-read sequencing data.

Cue achieves versatile and performant structural variant calling and genotyping using a deep-learning approach.

ERnet is a deep learning-based software tool for automatic segmentation and classification of structures in the endoplasmic reticulum. ERnet is compatible with many fluorescence imaging modalities and can uncover subtle phenotypic changes.

A self-inactivating variant of the CVS-N2c rabies virus enables both retrograde viral tracing and transcriptomic analyses, thereby allowing a combination of circuit mapping and molecular studies.

Femtosecond laser microdissection enables transcriptomic analyses of single neurons based on their phenotype.

Light-field microscopy is extended to mesoscale fields of view, allowing calcium imaging of thousands of neurons at a high frame rate and high spatial resolution.

A lightweight three-photon miniature microscope allows imaging neuronal activity throughout the cortex in freely moving mice.

A three-photon miniature microscope with optimized light-collection efficiency facilitates imaging of neuronal activity throughout the cortex, as well as in the hippocampus, in freely moving mice.