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An atlas of transcription factor expression during C. elegans development
A single-cell atlas of transcription factors mapped onto C. elegans cell lineage information reveals mechanisms of fate patterning and regulation during embryogenesis.
Professional editing can be a rewarding career. Here we discuss what the job entails, highs and lows, how to prepare for this career, the interview process and more.
Life scientists in Africa have had limited opportunity to participate in international advanced scientific training programs and workshops, which largely benefit researchers in North America, Europe and the Asia–Pacific region. Here, we chronicle the strategies adopted and challenges encountered in organizing Imaging Africa, an all-expenses-paid, continent-wide practical workshop in optical microscopy hosted in South Africa from 13 to 17 January 2020. Our experience can help steer other groups who similarly seek to organize impactful and sustainable training initiatives in Africa.
A four-dimensional single-cell atlas of transcription factor expression in Caenorhabditis elegans allows the identification of novel regulators of embryo development and the generation of molecular models of cell fate specification.
A combined experimental and computational approach to transcriptomic profiling of cell ‘multiplets’ enables the reconstruction of cell–cell interactions and higher-order structural features of biological tissues.
A protein expression atlas of transcription factors charted onto cell lineage maps of Caenorhabditiselegans development that uncovers mechanisms of spatiotemporal cell fate patterning and regulators of embryogenesis.
CIM-seq offers an unsupervised deconvolution method to profile cell–cell interactions by sequencing cell multiplets of a given tissue, and was employed to analyze diverse tissues such as the intestinal epithelium, lung and spleen.
e2gmm uses a deep neural network with a Gaussian representation to resolve the compositional and conformational variability within biomolecules using cryo-EM data.
Paint server and homology optimization pipeline (PaintSHOP), an interactive platform for the design of oligo fluorescence in situ hybridization (FISH) experiments, democratizes and standardizes the process of designing complex probe sets for the oligo FISH community.
Acoustic reporter genes can be imaged with high sensitivity using the BURST imaging sequence, allowing the detection of single cells under optimal conditions.
RAPID (rapid autofocusing via pupil-split image phase detection) is a sample-agnostic real-time autofocus method for widefield microscopy. RAPID removes most image degradation in large, cleared samples for enhanced quantitative analyses.
LiftPose3D infers three-dimensional poses from two-dimensional data or from limited three-dimensional data. The approach is illustrated for videos of behaving Drosophila, mice, rats and macaques.