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vLUME is a complete virtual reality environment for visualizing, analyzing and interacting with three-dimensional single-molecule localization microscopy data.
CRISPRi activities are measured from dCas9 fusions with 57 KRAB domains, which lead to the identification of ZIM3 KRAB–dCas9 fusion as a superior repressor.
The current standard approach for estimating error in proteome-scale cross-linking mass spectrometry datasets has severe limitations. A proposed set of data-quality metrics provides a more accurate assessment of error rate.
An open-source software platform, Genuage, enables the easy perception of, interaction with and analysis of multidimensional point clouds in virtual reality.
Feature-based molecular networking allows the generation of molecular networks for mass spectrometry data that can recognize isomers, incorporate relative quantification and integrate ion mobility data.
A systematic evaluation shows that excitation intensity has a dramatic impact on image quality in localization microscopy and reveals the benefits of lower excitation intensity for improved labeling efficiency and localization precision.
Repository-scale reanalysis of public mass spectrometry-based metabolomics data is facilitated by the Reanalysis of Data User (ReDU) interface, a system that uses consistent formatting and controlled vocabularies for metadata capture.
Stimulated Brillouin scattering microscopy overcomes the trade-off between acquisition speed and spectral resolution in spontaneous Brillouin scattering microscopy and allows visualization of elasticity and viscosity, as shown in C. elegans.
Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.
CARPID uses CRISPR technology to navigate biotin ligase to specific lncRNAs, which allows proximal labeling and thus the querying of RNA–protein interactions in living cells.
Split-FISH leverages a split-probe design to achieve enhanced specificity in multiplexed fluorescence in situ hybridization. Split-FISH reduces background and false positives, enabling transcriptome profiling in uncleared tissues.
Sorting RT-FDC combines real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves to transfer molecular specificity to label-free, image-based cell sorting using an efficient deep neural network.
Theoretical and experimental guidelines to determine laser intensity for single-photon excitation in microcrystals for ultrafast pump–probe experiments by serial femtosecond crystallography.
jYCaMP1, a yellow variant of the calcium indicator jGCaMP7, enables fast multicolor two-photon imaging at excitation wavelengths above 1,000 nm for use with popular ytterbium-doped fiber and modelocked semiconductor lasers.
Structural and biochemical insights help engineer a cytosine base editor variant that possesses improved on-target activity with minimal DNA and RNA off-target editing.
A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats.