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A two-step error correction process for high throughput–sequenced T- and B-cell receptors allows the elimination of most errors while not diminishing the natural complexity of the repertoires.
Light-activated reversible inhibition by assembled trap (LARIAT) is a versatile optogenetic method for inactivating proteins, including GFP fusions, in living cells by conditional clustering at high spatiotemporal resolution.
Noise models based on the identification of major sources of technical variability in single-cell RNA-seq data allow the inference of true biological variability in cellular expression.
Graphene is in many ways an ideal sample support for cryo-electron microscopy, but its hydrophobicity prevents adsorption of protein from aqueous solution. Low-energy hydrogen-plasma treatment renders graphene hydrophilic and enables controlled adsorption of protein to its surface.
A graphical processing unit implementation of an efficient Bayesian-based multiview deconvolution method brings the resolution and contrast advantages of multiview deconvolution to more users of light-sheet fluorescence microscopy.
Adaptive optics microscopy using a de-scanned, laser-induced guide star and direct wavefront sensing allows high numerical aperture diffraction-limited imaging of fine dynamic structures deep in the intact living zebrafish brain.
An approach for quantifying the similarity between dynamic ensembles of biomolecular structures is described and applied to RNA ensembles studied by nuclear magnetic resonance spectroscopy and molecular dynamics simulations.
Single-photon Airy beam excitation in light-sheet fluorescence microscopy combined with a simple deconvolution allows high-contrast imaging over a large field of view and minimal redundancy in illumination.
Methods are described for preparing unbiased libraries for transcriptome profiling of cells sorted according to the abundance of a transcript of interest.
A computational approach and software tool, cctbx.xfel, enables the determination of accurate macromolecular structure factors using a relatively small number of serial femtosecond crystallography diffraction snapshots.
Biomolecular interactions are directly detected and visualized in solution with a single-molecule method that measures time-dependent diffusion coefficient and mobility of electrokinetically trapped species.
CS-Rosetta-RNA is a web tool for determining high-resolution structures of noncoding RNA motifs using 1H NMR chemical shift data coupled with Rosetta-based modeling.
The combination of stimulated Raman scattering (SRS) microscopy with an alkyne group as a Raman-active tag allows high-contrast, live-cell dynamic imaging of a variety of biomolecules including DNA, RNA, protein, lipids and small-molecule drugs.
Multivariate linear mixed models implemented in the GEMMA software package add speed, power and the ability to test for genome-wide associations between genetic polymorphisms and multiple correlated phenotypes.
The synthetic firefly luciferase substrate CycLuc1 offers brighter bioluminescence and improved imaging in mouse models at lower doses than the standard D-luciferin.